Our customer and technical support experts are here to help! The retroviral ribonuclease H (retroviral RNase H) is a catalytic domain of the retroviral reverse transcriptase (RT) enzyme. ImProm-II™ Reverse Transcriptase. Check your inbox to complete email verification. It can be used to synthesize first strand cDNA at higher temperatures than the wild-type M-MuLV. The DNA polymerase domain occupies ~55% of the RT protein N-terminal. Trademarks There was an issue resetting your password. Although many researchers are successful in using M-MLV RT (H+) for analytical and some preparative cDNA applications, reverse transcriptases lacking RNase H activity provide another option to prepare long cDNAs and libraries containing a high percentage of full-length cDNA. Enter your username and we'll send a link to reset your password. Two bound manganese ions are shown as purple spheres. [5] A password reset email has been sent to the primary email address associated with your account. We offer numerous convenient solutions to meet your lab's needs. Himmel DM, Sarafianos SG, Dharmasena S, Hossain MM, McCoy-Simandle K, Ilina T, et al.

POLITIQUE DE CONFIDENTIALITE ET DEMANDES DE RENSEIGNEMENTS, Conditions générales de vente - Promega France, Supplied at a concentration of 100–200u/μl. Veuilez complèter votre compte à partir du mail que vous venez de recevoir, Vous ne pourrez pas avoir acès a votre compte si le mail n'est pas vérifié, Un compte existe déjà avec cette adresse email. Your password reset link has expired. Trademarks M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant. Legal and Trademarks Vous venez de créer votre compte Promega. A5003, A5004, … Our website uses functional cookies that do not collect any personal information or track your browsing activity. [11] The retroviral RNase H is needed to digest the tRNA molecule when it is no longer needed. Un problème est survenu lors de l'envoi de l'email de vérification. Erreur est survenue lors de la réinitialisation du mot de passe, Erreur de vérification du courrier électronique, M-MLV Reverse Transcriptase, RNase H Minus Protocol. En créant un compte, vous acceptez les termes et les conditions de la politique de confidentialité.

Connectez-vous avec votre nouveau mot de passe. By creating an account, you confirm that you accept the, M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant, M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant Protocol. Note: You will not be able to access your account until your email is verified. Congratulations! Himmel DM, Maegley KA, Pauly TA, Bauman JD, Das K, Dharia C, et al. We offer numerous convenient solutions to meet your lab's needs. When you select your country, you agree that we can place these functional cookies on your device. POLITIQUE DE CONFIDENTIALITE ET DEMANDES DE RENSEIGNEMENTS Please try again or contact Customer Service. Optimized, dependable reverse transcription. A bound inhibitor molecule, beta-thujaplicinol, is shown in green. Pour protéger votre vie privée, votre compte sera verrouillé après 6 tentatives infructueuses. A notable difference between the various RNase H proteins is the presence or absence of the C-helix (present in E. coli, MLV and human RNases H, absent in HIV-1, B. halodurans and ASLV RNases H), a positively charged alpha helix also referred to as the basic loop or protrusion. M-MLV Reverse Transcriptase. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained.

We've detected that you are using an older version of Internet Explorer. Enzyme for cDNA synthesis from long (<5kb) RNA transcripts. Mol. Recherche et développement de molécules cibles, Information sur l'utilisation des produits. Le mot de passe a été utilisé trop récemment.

Legal and Trademarks Storage Buffer: 20mM Tris-HCl (pH 7.5 at 25°C), 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet® P-40 and 50% glycerol. M5301. Cell. The four active-site carboxylate residues are shown in magenta. The RT enzyme is used to generate complementary DNA (cDNA) from the retroviral RNA genome. After that, you will need to contact Customer Service to unlock your account. Stay notified of Promega events, products and news. The RT enzyme is used to generate complementary DNA from the retroviral RNA genome.This process is called reverse transcription.To complete this complex process, the retroviral RT enzymes need to adopt a multifunctional nature. Terms and Conditions Félicitations! 2007 Use for double-stranded cDNA synthesis from RNA and subsequent ligation into a suitable vector. Identification des lignées cellulaires (STR), Méthodes automatisées pour la purification d'Acides nucléiques, Calculatrices + Outils d'analyse de données, How to Choose the Right Reverse Transcriptase.

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Improved Speed and Sensitivity in RT-LAMP RT-LAMP reactions with Bst 2.0 WarmStart DNA Polymerase and the indicated reverse transcriptase were incubated at 65°C with 1 pg - 100 ng of Jurkat total RNA. Un problème s'est produit lors de la vérification de votre adresse e-mail. Enzyme for full-length cDNA synthesis for the analysis of rare or long messages. SuperScript III RT offers higher cDNA yields, improved Veuillez réessayer ou contacter le Service Client. Un problème est survenu lors de la réinitialisation de votre mot de passe. They therefore possess 3 of the following biochemical activities: RNA-dependent DNA polymerase, ribonuclease H, and DNA-dependent DNA polymerase activities [2] ). Merci d'avoir vérifié votre adresse email. Veuillez réessayer ou contacter le service client. Amplifiable total RNA from FFPE tissues without organic solvents or overnight digestion.

There was an issue creating your account. The ribonuclease H domain from the HIV-1 reverse transcriptase protein. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus. Helpful for preparing long cDNAs and libraries with a high percentage of full-length cDNA. Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) that was created by introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability. This process is called reverse transcription.

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