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Calculate the appropriate resuspension volume for your oPools product with the IDT Resuspension Calculator . ���ljN #G�Z�]\3����ZM�>�1�:���>|d��S=!/")jx��\��_v��fd ��9�$kzg��ɿ��:V��Wi��BP'�(7��2���2�'�a�̃��gÛ�BǎH ɤ1��"�O5�G�֭/���u���O�R�9G�Έ�>a��k2a�~?vJT�UGH>��煰$�"�0���%é�1f)b �,��@�d_G������V2��N٘���+����d��K��?�(��8\�7t��,
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���#@йQ��;�����D�|nR�TϬ��ot3�?+*? © 2020 Integrated DNA Technologies. Email: sequence@functionalbio.com. Assays consist of a forward primer, a reverse primer, and a qPCR probe all delivered in a single tube or plate well. Resuspension calculator This calculates the volume needed to resuspend an oligo, given … Thevenin Termination Calculator. b� 6��V�b,���-#�t�y����|0 ��
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PC Clock Tools (MS Excel Spreadsheet) External Loop Filters Solver PLL External Loop Filter Calculator. integrations, User guides &
All assays were run in triplicate on the 7900 Real-Time PCR System (Thermo Fisher) using TaqMan® Gene Expression Master Mix (Thermo Fisher) under standard cycling conditions for 45 cycles. Tool, gBlocks Gene
Figure 5. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search. Enter your email below and click go! 525-01/02 Calculator 525-03 Calculator 525-04 Calculator 307-02 Calculator. * ZEN/Iowa Black™ FQ is a Double-Quenched Probe, which provides superior performance compared to traditional single-quenched probes. How much cDNA is recommended per PrimeTime qPCR Assay reaction? The standard curves were generated by running the assay with the indicated commercial master mixes. generation sequencing, Genes &
Figure 1. Most labs use inexpensive spectrophotometers that are focused on accessibility and ease of use. Solutions Calculator: Molecular Weight to Molarity. Libraries were prepared using 100 ng of human gDNA (NA12878, Coriell) using full-length UDI-UMI adapters (IDT) or amplified using 4 cycles of PCR and sequenced on the MiSeq ® (PCR-free) and HiSeq ® 4000 (amplified) platforms (Illumina). Click on the desired Timing Calculator link below: 525-01/02 Calculator525-03 Calculator525-04 Calculator307-02 Calculator, M & N CalculatorIDT PLL Loop Filter Calculator. Alt-R Predesigned Cas9 crRNA Selection Tool, Order status (Genes & gene fragments only), Target Capture Probe Design & Ordering Tool, Tips for resuspending and diluting your oligonucleotides, Calculations: Converting from nanograms to copy number. Molecular Biology Dilution & Solution Calculators, Resuspension Calculator: Moles to Molarity, Solutions Calculator: Molecular Weight to Molarity. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search. The first four assays were run on the 7900 Fast Real-Time PCR System (Thermo Fisher) and the final assay (Cy5) was run on the LightCycler® 480 System (Roche). cm [1]. Join thousands of engineers who never miss out on learning about the latest product technology. Figure 4. The data demonstrate greater than 90% efficiency and correlation coefficients greater than 0.99 for all tested qPCR master mixes. Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Tool, gBlocks Gene
Demonstrated assay performance with multiple dye–quencher combinations. Before you submit a part request, we kindly ask that you login or register to validate your email account. Thad Lane, BS, Manager of Process Engineering, IDT. Tool, gBlocks Gene
Create your free account today and enjoy unlimited access to our innovative web tools, streamlined ordering, and expert educational content. Use this formula to calculate the number of copies of your DNA sequence when moles are provided. Contact Technical and Customer Support for assistance. The data illustrates robustness in PCR efficiency and R2 values close to one across all dye/quenchers available for PrimeTime Assays. genomics, GMP, OEM &
Dilute solution to a desired Molarity. Entry, PrimerQuest
Fragments Entry. Understanding melt curves for improved SYBR Green assay analysis and troubleshooting. 505 South Rosa Road, Suite 238 | Madison, WI 53719, Phone: (608) 441-8125 / (877) 293-7947 | Fax: (608) 441-8127
Generally speaking, the greater the transfer volume, the more accurate and precise transfer will be. ©2020 Functional Biosciences, Inc. All Rights Reserved. A minimum order of 24 assays is required per plate. 3 Easy steps for determining oligonucleotide extinction coefficient and concentration. All reactions were run with TaqMan® Gene Expression Master Mix (Thermo Fisher) under standard cycling conditions. sheets, Supplier
PrimeTime qPCR Probe Assays are offered in 3 different sizes to meet the needs of any qPCR experiment. * Predesigned assays are available for human, mouse, and rat targets. Unfortunately, researchers often have to sacrifice performance for speed. custom oligonucleotides, oligo modifications, and oligo handling. Available in various sizes, premixed, and shipped dried down. IEEE 1588 and Synchronous Ethernet Clocks, PCI Express® Clock Buffers and Multiplexers, Clocks - Extreme Performance (<150 fs RMS), Jitter Attenuators with Frequency Translation, 3.3V CBTLV Double Density (General Purpose Bus Switch), 3.3V QuickSwitch (High Bandwidth Bus Switch), Optical Transimpedance Amplifiers (TIA) - Datacom, Optical Transimpedance Amplifiers (TIA) - Telecom, Time Slot Interchange (TSI) Digital Switches. information, Webinars & video
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