base editing: precision chemistry on the genome and transcriptome of living cells

Grünewald J, Zhou R, Garcia SP, Iyer S, Lareau CA, Aryee MJ, Joung JK. CAS Article Google Scholar 20.

Journals. BIGpre: a quality assessment package for next-generation sequencing data. Nat.

these 2 base editor variants were more precise at their target sites by producing Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion.

Rev. Contact us The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data.

Expanding the genome-targeting scope and the site selectivity of high-precision base. 9.

2018 Dec;19(12):801. doi: 10.1038/s41576-018-0068-0. 144.208.68.136. Citations are the number of other articles citing this article, calculated by Crossref and updated daily.

by Nature Reviews Genetics, Base editing: precision chemistry on the genome and transcriptome of living cells, Base editing: precision chemistry on the genome and transcriptome of living cells, Published online: 15 October 2018; doi:10.1038/s41576-018-0059-1.

ACS Chem Biol 13(2):383–388, Ebert AD, Liang P, Wu JC (2012) Induced pluripotent stem cells as a disease modeling and drug screening platform. Fast and accurate long-read alignment with Burrows-Wheeler transform.

DNA base editors comprise a catalytically disabled nuclease fused to a…, Screening of CRISPR/Cas base editors to target the AMD high-risk Y402H complement factor H variant, Deleterious in late life mitochondrial alleles and aging: secrets of Japanese centenarians, Systematic perturbation of yeast essential genes using base editing, The KT Jeang Prize 2019: Reuben S. Harris, A reversible KO model reveals therapeutic potentials of defective Tregs, Engineering plant virus resistance: from RNA silencing to genome editing strategies, Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations, Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Tong Y, Weber T, Lee SY (2018) CRISPR/Cas-based genome engineering in natural product discovery. Practitioners (doctors, other healthcare professionals), Science communicators (journalists, bloggers, editors), Biochemistry, Genetics and Molecular Biology. Please note: If you switch to a different device, you may be asked to login again with only your ACS ID. Ya-Ju Chang and Christine L. Xu contributed equally to this work and are co-first authors. 2020, Received: Rees HA, Liu DR (2018) Base editing: precision chemistry on the genome and transcriptome of living cells. This article is cited by Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. | Cancer Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. J Cell Physiol 226(5):1149–1156, Kwon DJ et al (2017) Effects of cell cycle regulators on the cell cycle synchronization of porcine induced pluripotent stem cells. This is a preview of subscription content. fewer multiple C edits. By continuing you agree to the, https://doi.org/10.1016/j.molcel.2020.07.005, Rationally Designed APOBEC3B Cytosine Base Editors with Improved Specificity, Download Hi-res Base editors are tools that chemically modify the nucleobases of DNA and RNA in a programmable manner, allowing for genome, epigenome, and transcriptome editing in live cells.

Annu Rev Biochem 85:227–264, Dy RL et al (2014) Remarkable mechanisms in microbes to resist phage infections. Springer Nature is developing a new tool to find and evaluate Protocols. Base editors are tools that chemically modify the nucleobases of DNA and RNA in a programmable manner, allowing for genome, epigenome, and transcriptome editing in live cells. Part of Springer Nature. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Base editors are tools that chemically modify the nucleobases of DNA and RNA in a programmable manner, allowing for genome, epigenome, and transcriptome editing in live cells. Some features of the site may not work correctly.

Base editing: precision chemistry on the genome and transcriptome of living cells Overview of attention for article published in Nature Reviews Genetics, October 2018 Altmetric Badge

This one has done particularly well, scoring, Older research outputs will score higher simply because they've had more time to accumulate mentions. Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base.

Canu: scalable and accurate long-read assembly via adaptive. Learn more. Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Comments that are commercial or promotional in nature, pertain to specific medical cases, are not relevant to the article for which they have been submitted, or are otherwise inappropriate will not be posted. Three papers in this issue report new methods for monitoring gene-editing tools in vivo (see the Perspective by Kempton and Qi). image. Base editing: precision chemistry on the genome and transcriptome of living cells. Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice.

Author information: (1)Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA. If you already have an abstream.me, Facebook or Google account: to follow your favorite journals, and filter articles that match your interests, n/a https://doi.org/10.1016/j.celrep.2020.107723, https://doi.org/10.1371/journal.pone.0235102. July 2, Mol Hum Reprod 16(11):856–868, Malik N, Rao MS (2013) A review of the methods for human iPSC derivation. 2020, Coronavirus RNA Proofreading: Molecular Basis and Therapeutic Targeting, Structures of the Cmr-β Complex Reveal the Regulation of the Immunity Mechanism of Type III-B CRISPR-Cas, We use cookies to help provide and enhance our service and tailor content and ads.

These tools can be used to introduce specific base transitions in DNA or RNA, manipulate methylation patterns in the epigenome, and create genetically encoded libraries in target genes.

Annu Rev Virol 1(1):307–331, Chibani-Chennoufi S et al (2004) Phage-host interaction: an ecological perspective. These tools can be used to introduce specific base transitions in DNA or RNA, manipulate methylation patterns in the epigenome, and create genetically encoded libraries in target genes.

Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors. Methods Mol Biol 997:23–33, Kelaini S, Cochrane A, Margariti A (2014) Direct reprogramming of adult cells: avoiding the pluripotent state.

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Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. To read this article in full you will need to make a payment. In cells, this activity can lead to changes in DNA sequence or RNA transcript abundance. Not affiliated

Xiao Wang, Chengfeng Ding, Wenxia Yu, Ying Wang, Siting He, Bei Yang, Yi-Chun Xiong, Jia Wei, Jifang Li, Jiayi Liang, Zongyang Lu, Wei Zhu, Jing Wu, Zhi Zhou, Xingxu Huang, Zhen Liu, Li Yang, Jia Chen. Base editing: precision chemistry on the genome and transcriptome of living cells Holly A. Rees, David R. Liu RNA-guided programmable nucleases from CRISPR systems generate precise breaks in DNA or RNA at specified positions. Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. YJC and CLX wrote and edited the manuscript. This one has done well, scoring.

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You are currently offline. To submit a comment for a journal article, please use the space above and note the following: We use cookies to help provide and enhance our service and tailor content and ads. J Cardiovasc Pharmacol 60(4):408–416, Masip M et al (2010) Reprogramming with defined factors: from induced pluripotency to induced transdifferentiation.

Base editing: precision chemistry on the genome and transcriptome of living cells.

An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities. RNA-programmed genome editing in human cells, Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions, The new frontier of genome engineering with CRISPR-Cas9, Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery, In vivo base editing of post-mitotic sensory cells, Genome-wide target specificities of CRISPR RNA-guided programmable deaminases. Characterization of the catalytic domain of human APOBEC3B and the critical structural role for a conserved methionine.

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