Sugano SS, Shirakawa M, Takagi J, Matsuda Y, Shimada T, Hara-Nishimura I, Kohchi T. CRISPR/Cas9-mediated targeted mutagenesis in the liverwort Marchantia polymorpha L. Plant Cell Physiol.
Valton J, Dupuy A, Daboussi F, Thomas S, Marechal A, Macmaster R, Melliand K, Juillerat A, Duchateau P. Overcoming transcription activator-like effector (TALE) DNA binding domain sensitivity to cytosine methylation. In the latter study, a tool kit was developed for easy assembly of a final T-DNA construct for simultaneous transcriptional modulation at multiple genetic loci in plants [81]. Sci Rep. 2015;5:11491.

A rapid assay to quantify the cleavage efficiency of custom-designed nucleases in planta. I have been doing the same thing as explained in the following paper -. Demorest ZL, Coffman A, Baltes NJ, Stoddard TJ, Clasen BM, Luo S, Retterath A, Yabandith A, Gamo ME, Bissen J, et al. As an effective genome editing tool, TALEN has been applied to generate useful traits in crops. Mol Plant. Mutat Res. Hi! Disruption of the target DNA by Crispr-Cas+gRNA-only works well (up to 99% efficacy). Wang Q, Lu Y, Xin Y, Wei L, Huang S, Xu J. Genome editing of model oleaginous microalgae Nannochloropsis spp. In this work the authors employed homologous recombination of a vector with 1.1 and 4.3kb regions of homology (upstream and downstream of the integration site, respectively). Life is your talents discovered This talk was given at a local TEDx event, produced independently of the TED Conferences. 2016;7:377. Despite proven concept, there is almost no report on utilizing de novo-engineered TAL activators or repressors in plant research.
Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. The LAGLIDADG family consists of popular meganucleases I-CreI and I-SceI. 2014;42(17):10903–14. Mol Cell Biol. Please let me know if you have also has any issues too. Plant Cell Rep. 2016;35(7):1429–38. Sci Rep. 2016;6:26685. An Agrobacterium-delivered CRISPR/Cas9 system for high-frequency targeted mutagenesis in maize. Nat Biotechnol.

Peng A, Chen S, Lei T, Xu L, He Y, Wu L, Yao L, Zou X.

2015;16:144. Two sgRNA-Nickase and an ssOD were used for the knock-in.

clustered regularly interspaced palindromic repeats. Sci Rep. 2016;6:26871. In the tobacco protoplasts, about 30% of transformed cells had NHEJ mutations and 14% showed targeted insertion due to HDR [43]. Variation in the flowering gene SELF PRUNING 5G promotes day-neutrality and early yield in tomato. A defect in DNA Ligase4 enhances the frequency of TALEN-mediated targeted mutagenesis in rice.

This typically results in deletions as DNA between homologous sections are removed. 2017. doi:10.1111/nph.14510. Plant Mol Biol.

Ma L, Zhu F, Li Z, Zhang J, Li X, Dong J, Wang T. TALEN-based mutagenesis of lipoxygenase LOX3 enhances the storage tolerance of rice (Oryza sativa) seeds.


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