For the same protospacer targets, cleavage efficiencies of chimeric RNAs were either lower than those of crRNA:tracrRNA . Le Cong, 1,2 * F. Ann Ran, 1,4 * David Cox, 1,3. We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells. Science (New York, N.Y.) 2013-1-5 Multiplex genome engineering using CRISPR/Cas systems. [Le Cong, F Ann Ran, David Cox, Shuailiang Lin, Robert Barretto, Naomi Habib, Patrick D Hsu, Xuebing Wu, Wenyan Jiang, Luciano A Marraffini, Feng Zhang] We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells. Shuailiang Lin, 1,5. Multiplex Genome Engineering Using CRISPR/Cas Systems Le Cong,1,2* F. Ann Ran,1,4* David Cox,1,3 Shuailiang Lin,1,5 Robert Barretto,6 Naomi Habib,1 Patrick D. Hsu,1,4 Xuebing Wu,7 Wenyan Jiang,8 Luciano A. Marraffini,8 Feng Zhang1† Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. the CRISPR system in modifying different loci across multiple organ-isms (Table S1). Multiplex Genome Engineering Using CRISPR/Cas Systems . ... Multiplex Genome Engineering Using CRISPR/Cas Systems. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage.


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